Intermediate

Part:BBa_K2170114:Design

Designed by: Max Mustermann   Group: iGEM16_LMU-TUM_Munich   (2016-10-11)


Intermediate of biotin binding receptor and secretory BirA (IRES IgKappa_BirA StrepDEL_PolyA) in RFC


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal BglII site found at 1306
    Illegal BamHI site found at 374
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    INCOMPATIBLE WITH RFC[1000]
    Illegal BsaI site found at 149
    Illegal BsaI site found at 1854
    Illegal SapI site found at 306


Design Notes

none


Source

assembly

References